Perfusion-Based 3D Endometrial Organoid Culture in a Microfluidic Chip

To establish a dynamic three-dimensional culture system, endometrial organoids consisting of three different cell types were cultivated within a commercially available microfluidic chip and subjected to perfusion using a flow pressure controller. This approach aimed to mimic the physiological environment of the endometrium, facilitating the study of endometrial function and disease in vitro.