pH Buffer Solutions with Polyethylene Glycol and Jeffamine for Protein Crystallization

pH Buffer Solutions for Protein Crystallization

This list provides a comprehensive overview of pH buffer solutions commonly used in protein crystallization. Each entry details the buffer system, its concentration, pH, and the type and concentration of the precipitant used.

B Series

• B9: 0.1 M Tris pH 8.0, 30% v/v Polyethylene glycol 400* B11: 0.1 M MES monohydrate pH 6.0, 20% w/v Polyethylene glycol monomethyl ether 2,000

C Series

• C3: 0.1 M BIS-TRIS propane pH 9.0, 10% v/v Jeffamine® ED-2001 pH 7.0* C5: 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 18% w/v Polyethylene glycol 3,350* C6: 0.1 M HEPES pH 7.5, 12% w/v Polyethylene glycol 3,350* C8: 0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4,000* C9: 0.1 M Tris pH 8.0, 28% w/v Polyethylene glycol 4,000* C11: 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5,000* C12: 0.1 M BICINE pH 8.5, 8% w/v Polyethylene glycol monomethyl ether 5,000

D Series

• D2: 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol 6,000* D6: 0.1 M HEPES pH 7.5, 4% w/v Polyethylene glycol 8,000* D8: 0.1 M BIS-TRIS pH 6.5, 16% w/v Polyethylene glycol 10,000* D9: 0.1 M BICINE pH 8.5, 20% w/v Polyethylene glycol 10,000* D10: 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 18% w/v Polyethylene glycol 20,000* D11: 0.1 M Imidazole pH 7.0, 12% w/v Polyethylene glycol 20,000* D12: 0.1 M BIS-TRIS propane pH 9.0, 8% w/v Polyethylene glycol 20,000

Note:

• 'v/v' indicates volume/volume percentage.* 'w/v' indicates weight/volume percentage.* Jeffamine® is a registered trademark of Huntsman Corporation.

This list can be used as a starting point for optimizing protein crystallization conditions. For best results, experiment with different combinations of buffers, precipitants, and pH values to find the optimal conditions for crystal growth.