以下是包含高浓度聚乙二醇 (PEG) 和 Jeffamine® 的缓冲液配方,以及相应的 pH 值和浓度。

B 组

B9: 0.1 M BICINE pH 8.5, 15% w/v Polyethylene glycol 1,500 B11: 0.1 M MES monohydrate pH 6.0, 20% w/v Polyethylene glycol monomethyl ether 2,000

C 组

C3: 0.1 M BIS-TRIS propane pH 9.0, 10% v/v Jeffamine® ED-2001 pH 7.0 C5: 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 18% w/v Polyethylene glycol 3,350 C6: 0.1 M HEPES pH 7.5, 12% w/v Polyethylene glycol 3,350 C8: 0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4,000 C9: 0.1 M Tris pH 8.0, 28% w/v Polyethylene glycol 4,000 C11: 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5,000 C12: 0.1 M BICINE pH 8.5, 8% w/v Polyethylene glycol monomethyl ether 5,000

D 组

D2: 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol 6,000 D6: 0.1 M HEPES pH 7.5, 4% w/v Polyethylene glycol 8,000 D8: 0.1 M BIS-TRIS pH 6.5, 16% w/v Polyethylene glycol 10,000 D9: 0.1 M BICINE pH 8.5, 20% w/v Polyethylene glycol 10,000 D10: 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 18% w/v Polyethylene glycol 20,000 D11: 0.1 M Imidazole pH 7.0, 12% w/v Polyethylene glycol 20,000 D12: 0.1 M BIS-TRIS propane pH 9.0, 8% w/v Polyethylene glycol 20,000

缓冲液配方:高浓度聚乙二醇(PEG)和 Jeffamine® 溶液

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