Engineered Promoters: Achieving Stable Gene Expression at Varying Copy Numbers in Bacteria

This research investigates a critical issue in the field of gene engineering: ensuring consistent gene expression levels across different copy numbers. Traditional gene expression regulation often relies on copy number, meaning more copies result in higher expression. However, copy numbers can fluctuate due to cell division or gene transfer, leading to unstable gene expression and hindering practical applications.

To address this problem, researchers have developed 'Engineered promoters', which are artificially designed and optimized promoter sequences. These promoters enable stable gene expression regardless of the number of gene copies. This study provides a novel solution for gene engineering, enhancing the stability and controllability of gene expression, thereby advancing the development and application of this technology.