Exploring the Proteome of Boar Sperm: Investigating Factors Influencing Semen Preservation Ability

Semen quality is paramount in animal breeding and reproduction. The widespread adoption of artificial insemination in the pig industry has escalated the demand for superior boar semen. A hallmark of high-quality boar semen is its robust preservation capability. However, the assessment of semen storage potential remains challenging in practical settings. Consequently, delving into the mechanisms underlying boar semen preservation is of paramount importance. To unravel potential biological insights connected to boar semen preservation, this research undertook a proteomic analysis of sperm samples derived from two groups of boars, GRE and PRE, exhibiting distinct liquid preservation capabilities at 17°C. Notably, sperm and seminal plasma proteins are pivotal determinants of semen preservation capacity. Existing research underscores that seminal plasma exerts a minimal influence on semen quality variations within the initial three days post-ejaculation. The selected semen samples from both groups in this study, evaluated for their quality parameters (TM and PM) after a three-day storage period at 17°C, demonstrated significant differences. This observation, to a certain degree, diminishes the interference of seminal plasma on semen preservation capacity, thereby enhancing the reliability of the research findings pertaining to sperm proteins.