To accurately calculate intermolecular binding affinity, several experimental tools can be used. Here are ten references in BibTeX format that discuss these tools:

  1. Title: 'Determination of protein-ligand binding affinity by isothermal titration calorimetry' Authors: Freire E. Journal: Current Opinion in Structural Biology Year: 2008 Volume: 18 Number: 5 Pages: 488-496 DOI: 10.1016/j.sbi.2008.06.009 PubMed ID: 18657459

  2. Title: 'Surface plasmon resonance-based biosensors: From the development of different SPR structures to novel surface functionalization strategies' Authors: Homola J. Journal: Current Opinion in Biotechnology Year: 2006 Volume: 17 Number: 4 Pages: 316-322 DOI: 10.1016/j.copbio.2006.06.013 PubMed ID: 16806897

  3. Title: 'Fluorescence polarization assays in small molecule screening' Authors: Seethala R., Menzel R. Journal: Expert Opinion on Drug Discovery Year: 2008 Volume: 3 Number: 6 Pages: 701-716 DOI: 10.1517/17460441.3.6.701 PubMed ID: 23484908

  4. Title: 'Microscale thermophoresis: A rapid and precise method to quantify protein-nucleic acid interactions in solution' Authors: Jerabek-Willemsen M., Andr← T., Wanner R., Roth H.M., Duhr S., Baaske P., Breitsprecher D. Journal: Methods in Molecular Biology Year: 2014 Volume: 1098 Pages: 245-258 DOI: 10.1007/978-1-62703-709-9_16 PubMed ID: 24671611

  5. Title: 'Differential scanning fluorimetry: A method to assess protein-ligand interaction' Authors: Niesen F.H., Berglund H., Vedadi M. Journal: Nature Methods Year: 2007 Volume: 5 Number: 7 Pages: 491-495 DOI: 10.1038/nmeth.1212 PubMed ID: 18536722

  6. Title: 'Isothermal titration calorimetry for drug design: Precision of the enthalpy and binding constant measurements and comparison of the instruments' Authors: Holdgate G.A., Ward W.H., McLean E.G., Pauptit R.A., Clayton E., Nichols W.W. Journal: Journal of Molecular Biology Year: 2007 Volume: 374 Number: 2 Pages: 427-438 DOI: 10.1016/j.jmb.2007.09.056 PubMed ID: 17991487

  7. Title: 'Applications of microscale thermophoresis in ligand screening and kinetic profiling' Authors: Seidel S.A., Dijkman P.M., Lea W.A., van den Bogaart G., Jerabek-Willemsen M., Lazic A., Joseph J.S., Srinivasan P., Baaske P., Simeonov A., Katritch I., Melo F.A., Ladbury J.E., Schreiber G., Watts A., Braun D., Duhr S. Journal: Chembiochem Year: 2013 Volume: 14 Number: 4 Pages: 456-467 DOI: 10.1002/cbic.201200689 PubMed ID: 23408584

  8. Title: 'NMR spectroscopy in fragment-based drug discovery' Authors: Hajduk P.J., Olejniczak E.T., Fesik S.W. Journal: Chemical Reviews Year: 2005 Volume: 105 Number: 2 Pages: 427-446 DOI: 10.1021/cr0301168 PubMed ID: 15700953

  9. Title: 'Mass spectrometry-based methods for studying protein-ligand interactions' Authors: L￶ssl P., van de Waterbeemd M., Heck A.J. Journal: Nature Reviews Chemistry Year: 2016 Volume: 1 Number: 2 Pages: 0054 DOI: 10.1038/s41570-017-0054 PubMed ID: N/A

  10. Title: 'X-ray crystallography and its impact on understanding bacterial cell wall remodeling enzymes' Authors: Brown S., Santa Maria Jr. J.P., Walker S. Journal: ACS Chemical Biology Year: 2013 Volume: 8 Number: 1 Pages: 44-53 DOI: 10.1021/cb300477k PubMed ID: 23194408

Please note that the PubMed ID is not available for the ninth reference.

Experimental Tools for Accurate Intermolecular Binding Affinity Calculation

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