土壤细菌和真菌群落结构分析：基于16S rRNA和ITS1基因测序

Analysis of Soil Bacterial and Fungal Community Structure

Following the methods of Yang Guangzhu et al. [26], soil samples were collected on August 15, 2021, from both treatment and control groups at a depth of 20-40 cm. Genomic DNA extraction and quality detection were performed using the OMEGA Soil DNA Kit (M5635-02) reagent kit. PCR amplification was conducted using specific primers for the bacterial 16S rRNA V3-V4 region and the fungal ITS1 region. The experiments were outsourced to Shanghai Meiji Biomedical Technology Co., Ltd. Sequencing regions and primers are shown in Table 1. The sequencing results were processed and clustered into operational taxonomic units (OTUs) at 97% similarity using the Silva database. Finally, rarefaction was performed using the QIIME feature-table rarefy function. The diversity, species structure, and community composition of soil bacteria and fungi were analyzed using IPM SPSS Statistics 26 software to determine their correlations.