CBM-Based ELISA for Polysaccharide Quantification: A Novel Approach Using Carbohydrate-Binding Modules

Quantification is crucial for the research and development of polysaccharides. Currently, quantifying polysaccharides remains a challenge, requiring simplicity and specificity. Enzyme-linked immunosorbent assay (ELISA) has proven to be a simple and specific quantification strategy using antibodies. However, obtaining antibodies for polysaccharides is complex. Alternatively, carbohydrate-binding module (CBM) exhibits the ability to specifically recognize and bind carbohydrates, and can be acquired efficiently. In this study, we verified the feasibility of a CBM-based ELISA for quantifying polysaccharides by replacing antibodies with CBM. To illustrate this, hyaluronic acid (HA) was used as an example.