nTreg Suppression Assay Results: PTC-0432253 & PTC-0432366 Dose-Dependent Suppression of T Cell Proliferation

Attached please check the results and raw data of the nTreg suppression assay. 4 ratios were performed in this round (nTreg:T=1:1/0.5:1/0.25:1/0.125:1). From the Treg expansion results, PTC-0432253 and PTC-0432366 showed no effects on the expression of Foxp3 compared with the DMSO group. Both PTC-0432253 and PTC-0432366 could increase the expression of IFN-gamma within Foxp3+ cells. However, PTC-0432366 showed a stronger effect compared to PTC-0432253. In terms of suppression activity, both compounds showed a dose-dependent suppression of T cell proliferation. The highest suppression activity was observed at a ratio of 1:1, with a decrease in T cell proliferation of approximately 80% for both compounds. The suppression activity gradually decreased as the ratio of nTreg to T cells decreased. In conclusion, both PTC-0432253 and PTC-0432366 showed no effects on the expression of Foxp3 but could enhance the expression of IFN-gamma within Foxp3+ cells. Additionally, both compounds demonstrated dose-dependent suppression of T cell proliferation, with the highest suppression activity observed at a ratio of 1:1.