ATAC-seq: A High-Throughput Sequencing Method for Epigenome Analysis in Human Development Research

Epigenetics plays a crucial role in the regulation of cellular differentiation and development. However, traditional techniques for analyzing the epigenome require a large number of cells. In recent years, high-throughput sequencing methods have rapidly advanced. One such technique is the assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq), which can identify open chromatin regions and regulatory sequences in the genome with as few as 500 cells. This article provides a comprehensive review of the advantages of ATAC-seq technology and its applications and prospects in human developmental research. The aim is to provide guidance and reference for studies related to human cells and embryonic development.