Glycopeptide Enrichment Methods: Advances and Key Points for Protein Glycosylation Analysis

The article discusses advancements in methods for enriching glycopeptides, which are peptides that have been modified with glycans (sugar molecules), for the analysis of protein glycosylation. The authors highlight developments made in the past decade in glycopeptide enrichment techniques, including lectin-based enrichment, hydrazide chemistry, and solid-phase extraction methods. These advancements have allowed for improved sensitivity and specificity in the analysis of protein glycosylation. The key points of the article include the importance of glycopeptide enrichment in studying protein glycosylation, the different enrichment methods that have been developed, and the benefits and limitations of each method.