Academic Writing Enhancement: Polishing an Immunohistochemistry Procedure Description

Immunohistochemistry\nThe paraffin-embedded tissues underwent antigen retrieval by microwaving in 0.01 M sodium citrate (pH 6) for 10 minutes after deparaffinization, hydration, and elimination of endogenous peroxidase activity. Following that, they were incubated overnight at 4 ﾰC with an antibody against CD8 (1:100, ab101500, Abcam, Cambridge, UK) or PD1 (1:300, ab137132, Abcam, Cambridge, UK). Subsequently, the slides were incubated with the corresponding secondary antibodies (Zhongshan Goldenbridge Biotechnology, Beijing, China). The number of CD8+ and PD1+ T cells in five nonoverlapping high-power fields (400ￗ) was tallied, and the average value was calculated.