Optimizing Medical SCI Academic Paper Writing: A Case Study

RT-qPCR analysis was quantified using SYBR® Green (Roche, Basel, Switzerland). The relative expression of PTGES3 was determined using the 2-ΔΔCt method with GAPDH serving as an internal reference. RT-qPCR was performed in triplicate using samples obtained from three independent experiments. The primers employed in this study were as follows: PTGES3 forward primer (5'-CAAATGATTCCAAGCATAAAAGAAC-3'), PTGES3 reverse primer (5'-GGTAAATCTACATCCTCATCACCAC-3'), and GAPDH forward primer (5'-GAGAAGGCTGGGGCTCATTT-3'), GAPDH reverse primer (5'-AGTGATGGCATGGACTGTGG-3') used for qPCR. All experiments involving tissue samples were repeated three times. Statistical analysis was performed using GraphPad Prism version 9.1.0 (GraphPad Software Inc., San Diego, CA, USA), and a significance level of P < 0.05 was considered to indicate statistical significance.