Dynamic 3D Culture of Endometrial Organoids in a Microfluidic Chip with Perfusion Control

A dynamic 3D culture system was established for triple-cell-type endometrial organoids embedded in a commercially available microfluidic chip and perfused with a flow pressure controller. This system allows for the controlled and continuous perfusion of culture medium, creating a more physiologically relevant environment for organoid growth and function. By embedding the organoids within the microfluidic chip, the system provides a three-dimensional space for cell growth and interaction, mimicking the natural environment of the endometrium. The flow pressure controller ensures consistent and adjustable fluid flow, facilitating nutrient delivery and waste removal, crucial for maintaining organoid viability and functionality. This system represents a significant advancement in the field of endometrial research, enabling the study of endometrial physiology and disease in a more realistic and controllable manner.