Perfusion of Triple-Cell-Type Endometrial Organoids in a Microfluidic Chip for Dynamic 3D Culture

To establish a dynamic 3D culture system, triple-cell-type endometrial organoids were introduced into a commercially available microfluidic chip and perfused with the aid of a flow pressure controller. This approach aims to create an innovative and advanced 3D culture platform that more closely mimics the physiological environment of the endometrium. By incorporating three distinct cell types into the organoids and subjecting them to controlled perfusion, this system offers a powerful tool for studying endometrial function and development in a more realistic setting.