Crystallization Buffer Recipes: Polyethylene Glycol and Jeffamine Solutions for Protein Crystallization

This document provides a list of common crystallization buffer recipes used in protein crystallization experiments. The recipes include various buffer systems, pH ranges, polyethylene glycol (PEG) concentrations, and Jeffamine concentrations. These recipes can serve as a starting point for your crystallization experiments, but it's important to adjust the conditions to optimize for your specific protein.

Polyethylene Glycol (PEG) Solutions

A Series

A1: 0.1' M Citric acid pH 3.5, 34% v/v Polyethylene glycol 200
A2: 0.1' M Sodium citrate tribasic dihydrate pH 5.5, 38% v/v Polyethylene glycol 200
A3: 0.1' M HEPES pH 7.5, 42% v/v Polyethylene glycol 200
A4: 0.1' M Sodium acetate trihydrate pH 4.5, 30% v/v Polyethylene glycol 300
A5: 0.1' M BIS-TRIS pH 6.5, 25% v/v Polyethylene glycol 300
A6: 0.1' M BICINE pH 8.5, 20% v/v Polyethylene glycol 300
A7: 0.1' M Sodium acetate trihydrate pH 4.0, 15% v/v Polyethylene glycol 400
A8: 0.1' M MES monohydrate pH 6.0, 22% v/v Polyethylene glycol 400
A9: 0.1' M Tris pH 8.0, 30% v/v Polyethylene glycol 400
A10: 0.1' M Sodium citrate tribasic dihydrate pH 5.0, 30% v/v Polyethylene glycol monomethyl ether 550
A11: 0.1' M Imidazole pH 7.0, 25% v/v Polyethylene glycol monomethyl ether 550
A12: 0.1' M BIS-TRIS propane pH 9.0, 20% v/v Polyethylene glycol monomethyl ether 550

B Series

B1: 0.1' M Sodium acetate trihydrate pH 4.0, 10% v/v Jeffamineﾮ M-600ﾮ pH 7.0
B2: 0.1' M MES monohydrate pH 6.0, 20% v/v Jeffamineﾮ M-600ﾮ pH 7.0
B3: 0.1' M Tris pH 8.0, 30% v/v Jeffamineﾮ M-600ﾮ pH 7.0
B4: 0.1' M Citric acid pH 3.5, 14% w/v Polyethylene glycol 1,000
B5: 0.1' M Sodium citrate tribasic dihydrate pH 5.5, 22% w/v Polyethylene glycol 1,000
B6: 0.1' M HEPES pH 7.5, 30% w/v Polyethylene glycol 1,000
B7: 0.1' M Sodium acetate trihydrate pH 4.5, 30% w/v Polyethylene glycol 1,500
B8: 0.1' M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol 1,500
B9: 0.1' M BICINE pH 8.5, 15% w/v Polyethylene glycol 1,500
B10: 0.1' M Sodium acetate trihydrate pH 4.0, 10% w/v Polyethylene glycol monomethyl ether 2,000
B11: 0.1' M MES monohydrate pH 6.0, 20% w/v Polyethylene glycol monomethyl ether 2,000
B12: 0.1' M Tris pH 8.0, 30% w/v Polyethylene glycol monomethyl ether 2,000

C Series

C1: 0.1' M Sodium citrate tribasic dihydrate pH 5.0, 30% v/v Jeffamineﾮ ED-2001 pH 7.0
C2: 0.1' M Imidazole pH 7.0, 20% v/v Jeffamineﾮ ED-2001 pH 7.0
C3: 0.1' M BIS-TRIS propane pH 9.0, 10% v/v Jeffamineﾮ ED-2001 pH 7.0
C4: 0.1' M Citric acid pH 3.5, 25% w/v Polyethylene glycol 3,350
C5: 0.1' M Sodium citrate tribasic dihydrate pH 5.5, 18% w/v Polyethylene glycol 3,350
C6: 0.1' M HEPES pH 7.5, 12% w/v Polyethylene glycol 3,350
C7: 0.1' M Sodium acetate trihydrate pH 4.0, 10% w/v Polyethylene glycol 4,000
C8: 0.1' M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4,000
C9: 0.1' M Tris pH 8.0, 28% w/v Polyethylene glycol 4,000
C10: 0.1' M Sodium acetate trihydrate pH 4.5, 30% w/v Polyethylene glycol monomethyl ether 5,000
C11: 0.1' M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5,000
C12: 0.1' M BICINE pH 8.5, 8% w/v Polyethylene glycol monomethyl ether 5,000

D Series

D1: 0.1' M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 6,000
D2: 0.1' M Imidazole pH 7.0, 20% w/v Polyethylene glycol 6,000
D3: 0.1' M BIS-TRIS propane pH 9.0, 30% w/v Polyethylene glycol 6,000
D4: 0.1' M Citric acid pH 3.5, 28% w/v Polyethylene glycol 8,000
D5: 0.1' M Sodium citrate tribasic dihydrate pH 5.5, 16% w/v Polyethylene glycol 8,000
D6: 0.1' M HEPES pH 7.5, 4% w/v Polyethylene glycol 8,000
D7: 0.1' M Sodium acetate trihydrate pH 4.5, 10% w/v Polyethylene glycol 10,000
D8: 0.1' M BIS-TRIS pH 6.5, 16% w/v Polyethylene glycol 10,000
D9: 0.1' M BICINE pH 8.5, 20% w/v Polyethylene glycol 10,000
D10: 0.1' M Sodium citrate tribasic dihydrate pH 5.0, 18% w/v Polyethylene glycol 20,000
D11: 0.1' M Imidazole pH 7.0, 12% w/v Polyethylene glycol 20,000
D12: 0.1' M BIS-TRIS propane pH 9.0, 8% w/v Polyethylene glycol 20,000

E Series

E1: 0.8' M Lithium sulfate monohydrate, 0.1' M Sodium acetate trihydrate pH 4.0, 4% v/v Polyethylene glycol 200
E2: 0.2' M Lithium sulfate monohydrate, 0.1' M Sodium citrate tribasic dihydrate pH 5.0, 26% v/v Polyethylene glycol 200
E3: 0.05' M Calcium chloride dihydrate, 0.1' M MES monohydrate pH 6.0, 45% v/v Polyethylene glycol 200
E4: 28% v/v 2-Propanol, 0.1' M BIS-TRIS pH 6.5, 3% v/v Polyethylene glycol 200
E5: 20% v/v Tacsimate pH 7.0, 0.1' M HEPES pH 7.5, 2% v/v Polyethylene glycol 200
E6: 10% v/v 2-Propanol, 0.1' M Sodium citrate tribasic dihydrate pH 5.0, 26% v/v Polyethylene glycol 400
E7: 0.2' M Ammonium acetate, 0.1' M Sodium citrate tribasic dihydrate pH 5.5, 24% v/v Polyethylene glycol 400
E8: 0.2' M Ammonium sulfate, 0.1' M BIS-TRIS pH 6.5, 18% v/v Polyethylene glycol 400
E9: 0.1' M HEPES pH 7.5, 40% v/v Polyethylene glycol 400
E10: 6% v/v 2-Propanol, 0.1' M Sodium acetate trihydrate pH 4.5, 26% v/v Polyethylene glycol monomethyl ether 550
E11: 1.8' M Ammonium sulfate, 0.1' M BIS-TRIS pH 6.5, 2% v/v Polyethylene glycol monomethyl ether 550
E12: 0.15' M DL-Malic acid pH 7.0, 0.1' M Imidazole pH 7.0, 22% v/v Polyethylene glycol monomethyl ether 550

F Series

F1: 0.1' M Succinic acid pH 7.0, 0.1' M BICINE pH 8.5, 30% v/v Polyethylene glycol monomethyl ether 550
F3: 0.1' M Sodium malonate pH 8.0, 0.1' M Tris pH 8.0, 30% w/v Polyethylene glycol 1,000
F5: 0.2' M L-Proline, 0.1' M HEPES pH 7.5, 24% w/v Polyethylene glycol 1,500
F6: 10% v/v 2-Propanol, 0.1' M BICINE pH 8.5, 30% w/v Polyethylene glycol 1,500
F7: 0.1' M Sodium chloride, 0.1' M BIS-TRIS propane pH 9.0, 25% w/v Polyethylene glycol 1,500
F9: 20% v/v 2-Propanol, 0.1' M MES monohydrate pH 6.0, 20% w/v Polyethylene glycol monomethyl ether 2,000
F11: 4.0' M Potassium formate, 0.1' M BIS-TRIS propane pH 9.0, 2% w/v Polyethylene glycol monomethyl ether 2,000
F12: 50% v/v Tacsimate pH 4.0, 0.1' M Sodium acetate trihydrate pH 4.5, 1% w/v Polyethylene glycol 3,350

G Series

G1: 0.10% w/v n-Octyl-b-D-glucoside, 0.1' M Sodium citrate tribasic dihydrate pH 5.5, 22% w/v Polyethylene glycol 3,350
G2: 2% v/v Tacsimate pH 7.0, 5% v/v 2-Propanol, 0.1' M Imidazole pH 7.0, 8% w/v Polyethylene glycol 3,350
G3: 2% v/v 1,4-Dioxane, 0.1' M Tris pH 8.0, 15% w/v Polyethylene glycol 3,350
G4: 18% v/v 2-Propanol, 0.1' M Sodium citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 4,000
G5: 6% v/v Tacsimate pH 6.0, 0.1' M MES monohydrate pH 6.0, 25% w/v Polyethylene glycol 4,000
G6: 0.2' M Magnesium formate dihydrate, 0.1' M Sodium acetate trihydrate pH 4.0, 18% w/v Polyethylene glycol monomethyl ether 5,000
G7: 2% v/v Polyethylene glycol 400, 0.1' M Imidazole pH 7.0, 24% w/v Polyethylene glycol monomethyl ether 5,000
G8: 0.2' M Sodium formate, 0.1' M BICINE pH 8.5, 20% w/v Polyethylene glycol monomethyl ether 5,000
G9: 4% v/v 2-Propanol, 0.1' M BIS-TRIS propane pH 9.0, 20% w/v Polyethylene glycol monomethyl ether 5,000
G10: 6% v/v Ethylene glycol, 0.1' M Citric acid pH 3.5, 10% w/v Polyethylene glycol 6,000
G11: 0.15' M Lithium sulfate monohydrate, 0.1' M Citric acid pH 3.5, 18% w/v Polyethylene glycol 6,000
G12: 10% v/v 2-Propanol, 0.1' M Sodium acetate trihydrate pH 4.0, 22% w/v Polyethylene glycol 6,000

H Series

H1: 0.2' M Sodium chloride, 0.1' M Sodium acetate trihydrate pH 4.0, 22% w/v Polyethylene glycol 8,000
H2: 20% v/v 2-Propanol, 0.1' M Tris pH 8.0, 5% w/v Polyethylene glycol 8,000
H3: 10% v/v Polyethylene glycol 200, 0.1' M BIS-TRIS propane pH 9.0, 18% w/v Polyethylene glycol 8,000
H4: 15% v/v 2-Propanol, 0.1' M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 10,000
H5: 0.4' M Sodium malonate pH 6.0, 0.1' M MES monohydrate pH 6.0, 0.5% w/v Polyethylene glycol 10,000
H6: 0.2' M Potassium sodium tartrate tetrahydrate, 0.1' M BIS-TRIS pH 6.5, 10% w/v Polyethylene glycol 10,000
H7: 5% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.1' M HEPES pH 7.5, 10% w/v Polyethylene glycol 10,000
H8: 0.2' M Ammonium acetate, 0.1' M Tris pH 8.0, 16% w/v Polyethylene glycol 10,000
H9: 5% v/v 2-Propanol, 0.1' M Citric acid pH 3.5, 6% w/v Polyethylene glycol 20,000
H10: 1.0' M Sodium malonate pH 5.0, 0.1' M Sodium acetate trihydrate pH 4.5, 2% w/v Polyethylene glycol 20,000
H11: 0.2' M Magnesium chloride hexahydrate, 0.1' M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 20,000
H12: 3% w/v Dextran sulfate sodium salt, 0.1' M BICINE pH 8.5, 15% w/v Polyethylene glycol 20,000

Note:

'M' denotes molarity.
'v/v' denotes volume per volume.
'w/v' denotes weight per volume.
Adjust the pH of the buffer to your desired value.
The provided recipes are just examples. You may need to adjust the concentrations of the buffer components and precipitants to optimize for your specific protein and crystallization conditions.
It's always recommended to test several different conditions to find the optimal crystallization conditions for your protein.

Crystallization Buffer Recipes: Polyethylene Glycol and Jeffamine Solutions for Protein Crystallization