以下列出了一些常见pH缓冲溶液的配方,包含不同缓冲剂和聚乙二醇浓度,方便您根据实验需求选择合适的溶液。

B系列

  • B9: 0.1 M Tris pH 8.0, 30% v/v Polyethylene glycol 400
  • B11: 0.1 M MES monohydrate pH 6.0, 20% w/v Polyethylene glycol monomethyl ether 2,000

C系列

  • C3: 0.1 M BIS-TRIS propane pH 9.0, 10% v/v Jeffamine® ED-2001 pH 7.0
  • C5: 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 18% w/v Polyethylene glycol 3,350
  • C6: 0.1 M HEPES pH 7.5, 12% w/v Polyethylene glycol 3,350
  • C8: 0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4,000
  • C9: 0.1 M Tris pH 8.0, 28% w/v Polyethylene glycol 4,000
  • C11: 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5,000
  • C12: 0.1 M BICINE pH 8.5, 8% w/v Polyethylene glycol monomethyl ether 5,000

D系列

  • D2: 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol 6,000
  • D6: 0.1 M HEPES pH 7.5, 4% w/v Polyethylene glycol 8,000
  • D8: 0.1 M BIS-TRIS pH 6.5, 16% w/v Polyethylene glycol 10,000
  • D9: 0.1 M BICINE pH 8.5, 20% w/v Polyethylene glycol 10,000
  • D10: 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 18% w/v Polyethylene glycol 20,000
  • D11: 0.1 M Imidazole pH 7.0, 12% w/v Polyethylene glycol 20,000
  • D12: 0.1 M BIS-TRIS propane pH 9.0, 8% w/v Polyethylene glycol 20,000

请注意,以上配方仅供参考,具体使用时应根据实验需求进行调整。


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