pH缓冲溶液配方:多种缓冲剂和聚乙二醇浓度
以下列出了一些常见pH缓冲溶液的配方,包含不同缓冲剂和聚乙二醇浓度,方便您根据实验需求选择合适的溶液。
B系列
- B9: 0.1 M Tris pH 8.0, 30% v/v Polyethylene glycol 400
- B11: 0.1 M MES monohydrate pH 6.0, 20% w/v Polyethylene glycol monomethyl ether 2,000
C系列
- C3: 0.1 M BIS-TRIS propane pH 9.0, 10% v/v Jeffamine® ED-2001 pH 7.0
- C5: 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 18% w/v Polyethylene glycol 3,350
- C6: 0.1 M HEPES pH 7.5, 12% w/v Polyethylene glycol 3,350
- C8: 0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4,000
- C9: 0.1 M Tris pH 8.0, 28% w/v Polyethylene glycol 4,000
- C11: 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5,000
- C12: 0.1 M BICINE pH 8.5, 8% w/v Polyethylene glycol monomethyl ether 5,000
D系列
- D2: 0.1 M Imidazole pH 7.0, 20% w/v Polyethylene glycol 6,000
- D6: 0.1 M HEPES pH 7.5, 4% w/v Polyethylene glycol 8,000
- D8: 0.1 M BIS-TRIS pH 6.5, 16% w/v Polyethylene glycol 10,000
- D9: 0.1 M BICINE pH 8.5, 20% w/v Polyethylene glycol 10,000
- D10: 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 18% w/v Polyethylene glycol 20,000
- D11: 0.1 M Imidazole pH 7.0, 12% w/v Polyethylene glycol 20,000
- D12: 0.1 M BIS-TRIS propane pH 9.0, 8% w/v Polyethylene glycol 20,000
请注意,以上配方仅供参考,具体使用时应根据实验需求进行调整。
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