Microbubble-Nanoliposomal Delivery System for Efficient and Targeted Hair Growth Regeneration

We aimed to improve the effectiveness and safety of delivering Cas9/sgRNA riboprotein complexes. Our team developed a carrier in the form of a microbubble-nanoliposomal particle, which can be targeted to a specific site with the help of ultrasound activation. This system effectively facilitates local delivery to a specific site when agitated by ultrasound activation. In practice, we successfully transferred the protein constructs into dermal papilla cells in the hair follicle of androgenic alopecia animals by microbubble cavitation induced sonoporation of our particle. The delivered Cas9/sgRNA recognized and edited specifically the target gene with high efficiency in vitro and in vivo, thus recovering hair growth. Through this technology, we were able to successfully transfer protein constructs to dermal papilla cells in hair follicles affected by androgenic alopecia. Furthermore, the Cas9/sgRNA riboprotein complex effectively recognized and edited the target gene with high efficiency both in vitro and in vivo, leading to a remarkable recovery of hair growth.