Enrichment Analysis and Identification of Key Genes Associated with IHF using ClusterProfiler

To conduct our analysis, we leveraged the clusterProfiler package to perform three types of enrichment analyses: Gene Ontology (GO), Kyoto Gene and Gene Encyclopedia (KEGG), and Gene Set Enrichment Analysis (GSEA). Specifically, GSEA was applied to all genes within our dataset. Next, we identified the key genes associated with IHF by intersecting differentially expressed genes (DEGs) with important module genes from the Weighted Gene Co-expression Network Analysis (WGCNA). To further investigate the functional relevance of these key genes, we conducted GO and KEGG enrichment analyses.