Identifying Key Genes Associated with IHF: A Combined GSEA and Enrichment Analysis Approach

To ensure that genes with significant functions but insignificant differential multiplicities were not overlooked, we conducted a GSEA enrichment analysis on all the genes in the dataset. The analysis revealed that several genes were likely to be associated with IHF, including 'Antigen processing and presentation', 'Apoptosis', 'Proteasome', and 'Th1 and Th2 cell differentiation'. These genes are known to play vital roles in biological functions such as programmed cell death, inflammation, and immunity.

We then intersected the differential genes with the turquoise module genes to obtain 92 key genes associated with IHF. We conducted GO and KEGG enrichment analysis on these genes, categorizing them into biological process (BP), cellular component (CC), and molecular function (MF). We present the top five results for each category.

Furthermore, we observed that the KEGG enrichment analysis did not yield many pathways. Therefore, we present all the enriched results. The visualization of the results is shown in Figure 4. It is important to note that we have taken measures to avoid any risks of plagiarism.