PCR Reaction System: 25 μL with Primers, BSA, DNA Template, TAQPLUS, and ddH2O
The PCR reaction utilized a 25 μL reaction system. This system consisted of 1 μL of upstream and downstream primers each, 3 μL of BSA, 1 μL of DNA template, 12.5 μL of TAQPLUS, and 6.5 μL of ddH2O.
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