Duroc Boar Semen Quality: A Comparative Study of Preservation Elasticity

A selection of 25 healthy and fertile Duroc boars, aged between one and three years, were chosen for this study, each undergoing a single semen collection. The collected semen was rigorously evaluated based on volume (≥100 ml), sperm concentration (≥200*10^6/ml), progressive motility (≥80%), abnormality rate (≤20%), and normal color and odor. Eighteen boars were initially selected and their semen was collected in triplicate. The first sample was used to isolate sperm and seminal plasma, subsequently analyzed through proteinomics. The second sample was utilized for evaluating sperm morphology. The third sample was diluted and stored at 17°C to monitor the decline in semen quality over time. Two individuals were removed from the study due to a significant drop in semen quality attributed to unstable external factors. Based on the total sperm motility and progressive motility index after seven days of storage, the remaining 16 boars were divided into two groups based on their extreme differences in preservation elasticity. The group exhibiting good preservation elasticity (GPE), comprising three boars, displayed the best semen quality after seven days of liquid storage at 17°C. Conversely, the group with poor preservation elasticity (PPE), also consisting of three boars, displayed the worst semen quality after seven days of storage at 17°C.