Proteomic Analysis Reveals Key Proteins Influencing Boar Sperm Preservation Elasticity

This groundbreaking study delves into the proteomic landscape of boar sperm, revealing novel insights into the factors influencing liquid preservation elasticity. The research underscores the pivotal role of proteins enriched in oxidative stress and cellular metabolic pathways in maintaining sperm viability and acquisition capability. Notably, proteins like O18994, P12309, and Q8WNV7, implicated in oxidative stress response, exhibit upregulation in the group with superior preservation elasticity. Conversely, A0A288CFV9 and A0A5G2Q7N8 display contrasting expression trends, highlighting their involvement in sperm tail assembly and vitality. Furthermore, a multitude of upregulated proteins, including F1S4S3, I3LH92, and F1S4Q8, participate in crucial metabolic processes and sperm acquisition. Drawing on prior research in sperm proteomics, the study identifies O18994 (seminal plasma), P12309, F1S4S3, F1S4Q8, A0A288CFV9, and Q863I2 as key proteins influencing boar sperm preservation capability. Q8WNV7, I3LG98, and F1RFD4 also demonstrate a significant role in this process. The study sheds light on the susceptibility of semen with poor preservation elasticity to early oxidative stress, leading to metabolic dysregulation, premature sperm acquisition, and accelerated apoptosis. These findings offer invaluable insights into the mechanisms governing boar sperm preservation, establishing a foundation for developing novel biotechnological strategies to assess sperm quality and optimize preservation methods.