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The Bardet-Biedl syndrome 7 protein homolog (A0A288CFV9), encoded by BBS7, is a pivotal player in ciliogenesis, demonstrably influencing sperm motility and morphology. This protein collaborates with GPR161, a protein encoded by IFT22 and F1RPV0, in the intricate process of flagellar assembly. However, the literature lacks conclusive evidence regarding the adverse effects of elevated BBS7 expression on semen quality. Further research is warranted to elucidate the precise role of BBS7 in semen. Moreover, the PRE group exhibits diminished expression of Q863I2 protein, encoded by OSR1. It has been documented that OSR1 regulates ion transport within the epididymis. Consequently, reduced OSR1 expression compromises sperm motility, resulting in impaired fertility due to disruptions in epididymal development and function. These findings align with the results of this study.