Boar Sperm Protein and Cold Preservation: A Proteomic Study

Preserving porcine semen at low temperatures is a vital aspect of the swine industry. Proteomic analysis offers significant potential for enhancing our understanding of this process. This study adopted an exploratory approach, selecting two groups of fresh boar ejaculate samples with contrasting elasticity and preserving them at 17°C. Seminal plasma was removed through centrifugation, followed by sperm protein mass spectrometry analysis. By examining changes in protein expression patterns between samples exhibiting varying cold preservation abilities at -17°C, we identified potential sperm proteins linked to liquid storage capacity, which could serve as promising biomarkers. This study provides a foundation for future research on this critical topic. To our knowledge, this is the first report establishing a connection between boar sperm protein and the semen's resistance to cold preservation at -17°C.