HPLC/MS-MS 测试条件 - C18 色谱柱，电喷雾电离

The HPLC/MS-MS test conditions are as follows: C18 chromatographic column (250 × 4.6mm, 3 μm), mobile phase A is formic acid solution (0.1%), mobile phase B is acetonitrile (0.1% formic acid), flow rate is 0.6 mL/min, injection volume is 10 μL, column temperature is 30℃, gradient run time is 20 min, and the specific mobile phase ratio settings are shown in Table 2-3. Electrospray ionization mode is used for mass spectrometry analysis, with an injection voltage of 3500 V, ion source temperature of 140℃, and nebulization temperature of 350℃.