微藻细胞数量响应曲面分析：ZM-3和ZM-5交互作用的影响

Fig. 3-2 presents the contour map of the number of algal cells, which shows the response of the protein content to the interaction effects of ZM-3 and ZM-5 at 6% inoculation rate. As depicted in the graphs, when ZM-3 is held constant, the quantity of algal cells gradually increases with the increase in ZM-5. On the other hand, when ZM-5 is held constant, the number of algal cells first increases and then decreases with the increase in ZM-value.

Under the influence of both factors, the number of algal cells reaches its maximum value within the selected range at ZM-3 (0.2-0.4) and ZM-5 (0.8-1.0). The response surface graph displays the interaction effect of ZM-3 and ZM-5 on the number of microalgal cells. It can be observed that the quantity of algal cells increases with the increase in ZM-5. The graph also highlights the significant interaction effect of both factors, where the response value of the number of microalgal cells is more sensitive to the changes in ZM-5, especially when ZM-5 is high and ZM-3 is low.

Furthermore, when ZM-5 is within the range of 0.8-1.0, the curve of ZM-5 is steeper, and the input of ZM-5 has a greater impact on the number of microalgal cells, particularly when ZM-3 is low. This implies that the higher proportion of ZM-5 input will increase the number of microalgal cells and contribute to the accumulation of microalgal cells. Therefore, the results suggest that the interaction effect of ZM-3 and ZM-5 is significant in determining the number of microalgal cells, and their proportion should be carefully considered to achieve optimal results.