缺血心肌能量代谢解析与MSCs优化移植策略研究

This project follows the research approach of 'Energy Metabolism Analysis - Microenvironmental Remodeling - Cell Remodeling - Large Animal Verification'. It aims to combine protein and metabolomics methods to deeply analyze the energy metabolism characteristics and molecular mechanisms of ischemic myocardium and transplanted MSCs. Based on this, potential intervention targets will be screened. On the one hand, the local microenvironmental metabolic characteristics and energy supply of ischemic myocardium will be actively changed, and on the other hand, ischemic adaptive MSCs will be transformed through genetic or pharmacological means in vitro. With the above optimal plan, pre-clinical large animal verification of MSCs cardiac transplantation will be conducted, and an MSCs optimization strategy will be established to enhance energy utilization efficiency and survival after in vivo transplantation, promote myocardial repair and regeneration.