缺血心肌能量代谢解析及MSCs移植优化策略研究

This project follows the research approach of 'energy metabolism analysis - microenvironment remodeling - cell remodeling - large animal validation'. It intends to combine protein and metabolomics methods to deeply analyze the energy metabolism characteristics and molecular mechanisms of ischemic myocardium and transplanted MSCs. Based on this, potential intervention targets will be screened. On the one hand, the local microenvironment metabolism characteristics and energy supply of ischemic myocardium will be actively changed. On the other hand, ischemic adaptive MSCs will be remodeled through gene or pharmacological means in vitro. With the above optimal scheme, the preclinical large animal validation of MSCs cardiac transplantation will be carried out, and the optimization strategy of MSCs will be established to enhance energy utilization efficiency and survival after in vivo transplantation, thus promoting myocardial repair and regeneration.