缺血心肌修复的能量代谢与干预策略：MSCs优化和临床前验证

This project follows the research approach of 'energy metabolism analysis-microenvironment remodeling-cell remodeling-large animal validation', and intends to combine protein and metabolomics methods to thoroughly analyze the energy metabolism characteristics and molecular mechanisms of ischemic myocardium and transplanted MSCs. Based on this, potential intervention targets will be screened. On one hand, the local microenvironment metabolism characteristics and energy supply of ischemic myocardium will be actively changed, while on the other hand, ischemic adaptive MSCs will be transformed through gene or pharmacological means in vitro. With the above optimization plan, the pre-clinical large animal validation of MSCs heart transplantation will be carried out, and an MSCs optimization strategy will be established to enhance energy utilization efficiency and survival after in vivo transplantation, promote myocardial repair and regeneration.