Enzymatic Hydrolysis of D-PL by D-Lac and its Variants: A Comparative Study
The hydrolytic efficacy of D-Lac and its various iterations on D-PL was evaluated in 25 mL triangular vials utilizing a 14 mL reaction system comprising 768 mM DL-PL, Tris-HCl buffer (500 mM, pH 7.5), and 200 uL of diluted purified enzyme. The reaction was allowed to proceed for 20 minutes at 30°C, after which the enzyme was inactivated through boiling and the resulting product was analyzed through reversed-phase high-performance liquid chromatography.
原文地址: https://www.cveoy.top/t/topic/kpcw 著作权归作者所有。请勿转载和采集!