Enzymatic Hydrolysis of D-Lac and its Derivatives on D-PL: A Comprehensive Study

The enzymatic hydrolysis of D-Lac and its derivatives on D-PL was assessed using a 14 mL reaction system in 25 mL triangular vials. This system comprised 768 mM DL-PL, Tris-HCl buffer (500 mM, pH 7.5), and 200 uL of purified enzyme. The reaction mixture was incubated at 30°C for 20 minutes, followed by enzyme inactivation through boiling. Subsequently, the reaction products were analyzed using reversed-phase high-performance liquid chromatography (HPLC).