Structural and Functional Analysis of D-Lac: Insights into D-Phenylglycine Biosynthesis

Figure 1 presents a multi-faceted analysis of D-Lac, a pivotal enzyme involved in the biosynthesis of D-phenylglycine. Utilizing AlphaFold2, we precisely predicted the structure of D-Lac, while Pointsite facilitated the identification of its binding pocket. Furthermore, sequence conservation analysis illuminated the functional significance of specific amino acid residues within D-Lac. The relative activities of the wild-type and its variants were meticulously determined, with specific activity turnover number and enantiomeric excess summarized in Table S4. Molecular docking was employed to simulate the binding of D-PL to D-Lac, revealing distinctive conformational preferences. Lastly, we investigated the dynamic nature of the D-Lac pocket, examining its 'open and closed' states to gain deeper insights into its functional mechanism. In summation, this figure provides a comprehensive and informative overview of D-Lac, highlighting its structural and functional characteristics and elucidating its crucial role in D-phenylglycine biosynthesis.