Attached please check the results and raw data of the nTreg suppression assay 4 ratios were performed in this round nTregT=11051025101251From the Treg expansion results PTC-0432253 and PTC-0432366 sho

Additionally, PTC-0432253 and PTC-0432366 also demonstrated a dose-dependent suppression of T cell proliferation. At a ratio of 1:1, both compounds significantly suppressed T cell proliferation compared to the DMSO group. As the ratio decreased to 0.5:1, 0.25:1, and 0.125:1, the suppressive effects of the compounds on T cell proliferation became even more pronounced.

In summary, PTC-0432253 and PTC-0432366 showed no effects on Foxp3 expression but increased IFN-gamma expression within Foxp3+ cells. Additionally, both compounds demonstrated a dose-dependent suppression of T cell proliferation. These results suggest that PTC-0432253 and PTC-0432366 have potential immunosuppressive effects and could be further investigated as potential therapeutic agents