For the ELISA results the release of IFN-gamma decreased with the increasing number of Tregs in DMSO group which is coordinated with the proliferation results And PTC-0432253 and PTC-0432366 have no e

This indicates that these compounds have the ability to enhance the production of IFN-gamma by T cells in the presence of Tregs. These results suggest that PTC-0432253 and PTC-0432366 may have potential immunomodulatory effects, specifically in promoting IFN-gamma release. Further studies are needed to elucidate the underlying mechanisms and to evaluate the therapeutic potential of these compounds in modulating the immune response.