如下内容的英文表达我觉得过于口语化能否用地道、科学、书面的英文表达并确保整体语言表达复合英语表达习惯:In order to investigate the reasons why HEO complex improves endometrial injury repair and fertility we observed the location of different time endom
To investigate the mechanisms underlying the beneficial effects of HEO complex on endometrial injury repair and fertility, we conducted a series of experiments in mice. Firstly, we examined the localization of different endometrial complexes at various time points and assessed the expression of human endometrial-specific markers. Following the injection of HEO and EO complexes for 3 days, we observed that the complexes containing cells and hydrogel remained relatively independent and filled the uterine cavity of the mice (Figure 7a). Immunostaining revealed the presence of human-specific CK7, Vimentin, and CD31 in the HEO graft, indicating the existence of human endometrial epithelial cells, stromal cells, and vascular endothelial cells. In contrast, the EO graft lacked the expression of human-specific CD31. After 7 days, the human endometrial epithelial cells in both HEO and EO complexes integrated into the mouse endometrium, with CK7-positive epithelial cells appearing in the subendometrial tissue (Figure 7a). These cells also expressed human-specific estrogen receptors and progesterone receptors (Figure 7b). These findings demonstrate that cells in the HEO complex contribute to the regeneration of damaged mouse endometrium, which correlates with the observed improvements in endometrial repair and fertility in the HEO group.
Furthermore, we subcutaneously injected the HEO complex into mice and retrieved the grafts after 14 days. Under stereomicroscopy, we observed well-developed blood vessels in the grafts (Figure 7c). By using human-specific fluorescent substances (UEA-1) and mouse-specific fluorescent substances, we confirmed the connection between the human and mouse blood vessel networks in the grafts (Figure 7d). Additionally, perfusion of fluorescein-conjugated dextran and UEA-1 revealed that the green-labeled human blood vessels contained red fluorescent substances, indicating perfusion of mouse blood within the human blood vessel network in the grafts. In contrast, the EO complex led to graft shrinkage and the formation of inflammatory vesicles. The formation of functional blood vessels by the HEO complex in mice may also contribute to the enhanced endometrial repair and improved fertility following transplantation, as graft blood vessel formation is crucial for graft success and functionality.
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