1 从人外周血单个核细胞中分离单核细胞诱导树突状细胞分化6天。利用LPS刺激树突状细胞24小时。2 在孔内加入树突状细胞和指定浓度9个剂量的测试单药以及与化疗药5-FU联用组在37℃下孵育24h。3 从人外周血单个核细胞中分离T细胞与树突状细胞共培养。4 第4天收集细胞进行流式分析检测T细胞增殖取上清进行IFN-γ和IL-2检测。用英文
- Monocytes were isolated from peripheral blood mononuclear cells and induced to differentiate into dendritic cells for 6 days. The dendritic cells were then stimulated with LPS for 24 hours.
- Dendritic cells, along with specified concentrations (9 doses) of test drugs and the combination with chemotherapy drug 5-FU, were added to wells and incubated at 37°C for 24 hours.
- T cells were isolated from peripheral blood mononuclear cells and co-cultured with dendritic cells.
- On day 4, cells were collected for flow cytometric analysis to evaluate T cell proliferation, and the supernatant was collected for IFN-γ and IL-2 detection
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