Quantification is crucial for the research and development of polysaccharides. Currently, the quantification of polysaccharides is still a challenging task, requiring simplicity and specificity. Enzyme-linked immunosorbent assay (ELISA) has been proven to be a simple and specific quantification strategy using antibodies. However, obtaining antibodies for polysaccharides is complex. On the other hand, carbohydrate-binding module (CBM) possesses the ability to specifically recognize and bind carbohydrates, and can be obtained efficiently. In this study, we verified the feasibility of a CBM-based ELISA for quantifying polysaccharides by replacing antibodies with CBM. To illustrate this, hyaluronic acid (HA) was used as an example

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