The tissue culture conditions for Salvia miltiorrhiza are broad, and various ratios of plant hormones (plant growth substances) can induce callus formation and regenerate plantlets within a certain range (Wang and Liu, 1987; Hu et al., 1992; Jiang, 1994; Zhao et al., 1999; Tian and Wang, 2003). Guo et al. (2007a, b) mainly studied the culture conditions for adventitious roots of S. miltiorrhiza, such as nitrogen source, carbon source, and organic compounds in the culture medium. Zhang et al. (1997) achieved crown gall tumor and hairy root regeneration in S. miltiorrhiza using Agrobacterium rhizogenes and Agrobacterium tumefaciens, but they did not introduce the target gene and did not involve resistance selection steps. These studies are important for tissue culture of S. miltiorrhiza, but systematic experiments, especially investigating the conditions for different callus induction using different plant hormones, have not been conducted yet, and these different callus types are of great significance for further research on S. miltiorrhiza. Regarding the genetic transformation system of S. miltiorrhiza, Wang (2005), Wang (2005), and Yan & Wang (2007) first reported an Agrobacterium-mediated genetic transformation system. They constructed vectors containing the target gene and transformed S. miltiorrhiza leaves with A. tumefaciens to obtain positive plants. Cui et al. (2015) also successfully transformed transgenic S. miltiorrhiza using an RNAi vector. However, due to different S. miltiorrhiza varieties, vectors, Agrobacterium strains, and other factors, our laboratory found that the results using existing methods in S. miltiorrhiza genetic transformation experiments were highly random, and often the transformed leaves of S. miltiorrhiza ceased to grow. Therefore, based on the existing literature, the author explored the conditions for inducing different types of callus from S. miltiorrhiza leaves and stem segments as explants, and systematically studied the genetic transformation system of S. miltiorrhiza using orthogonal design, optimizing a more reproducible genetic transformation system, providing more fundamental tools for the tissue culture research of S. miltiorrhiza

用科技论文的语言改写以下这些文字:丹参的组织培养条件宽泛一定范围内的植物激素 植物生长物质 配比均能诱导出愈伤并再生植株 王建英和刘涤 1987; 胡月红等 1992; 姜广奋1994; 赵洁等 1999; 田宇红和王喆之等2003 。郭肖红等 2007ab 主要从培养基的氮源、碳源及有机物等方面研究了丹参不定根的培养条件张荫麟等 1997 用发根农杆菌和根癌农杆菌转化丹参得到冠瘿瘤和毛状根再生得

原文地址: https://www.cveoy.top/t/topic/iGct 著作权归作者所有。请勿转载和采集!

免费AI点我,无需注册和登录