翻译：分子鉴定将PG-9菌株多次纯化利用16S rRNA基因引物以细菌总基因组DNA为模板进行PCR扩增经电泳检测回收PCR产物后委托北京六合华大基因有限公司进行分子鉴定得到了一条长度为1399 bp序列。经测序分析并与GenBank数据库进行比对发现PG-9与盖氏假单胞菌Pseudomonas gessardii相似性为9993MK8831341。进一步将测序结果构建系统发育树图3结果发现PG-

Molecular identification

The PG-9 strain was purified multiple times and PCR amplification was performed using 16S rRNA gene primers with bacterial genomic DNA as the template. After electrophoresis and recovery of the PCR products, molecular identification was conducted by Beijing Liuhe Huada Gene Co., Ltd. A sequence of 1399 bp in length was obtained. Sequencing analysis and comparison with the GenBank database revealed a similarity of 99.93% (MK883134.1) between PG-9 and Pseudomonas gessardii. Further analysis using the sequencing results to construct a phylogenetic tree (Figure 3) showed that the PG-9 strain clustered with P. gessardii. Combined with morphological identification, it was confirmed that the strain was P. gessardii with the GenBank accession number OP236515