帮我用英文美化这段话：Carbohydrate macroarray assays were performed with reference to McCartney’s method Ref The 2 mgmL of examined polysaccharides were spotted in triplicate onto nitrocellulose membranes GE Hea

Carbohydrate macroarray assays were conducted following McCartney's method [Ref]. The examined polysaccharides, at a concentration of 2 mg/mL, were carefully spotted onto nitrocellulose membranes (GE Healthcare, Uppsala, Sweden) in triplicate. After allowing them to dry at room temperature for 30 minutes, the membranes were then blocked with MPBS buffer (140 mM NaCl, 10 mM Na2HPO4, 2.7 mM KCl, and 1.7 mM KH2PO4, pH 7.5) containing 5% skim milk powder for a duration of 1 hour. Subsequently, the membranes were probed with either crude recombinant proteins (cell-free extracts) or purified recombinant proteins at a concentration of 50 μg/mL. Following a water wash, the membranes were incubated for 2 hours with an HRP-conjugated (His)6-tag monoclonal antibody (Proteintech, IL, USA). After another water wash, the membranes were developed for 2 minutes using an electrochemiluminescence solution.