帮我用英文重新撰写这段话：Carbohydrate macroarray assays were performed with reference to McCartney’s method Ref The 2 mgmL of examined polysaccharides were spotted in triplicate onto nitrocellulose membranes GE H

Carbohydrate macroarray assays were conducted following McCartney's method [Ref]. The examined polysaccharides, at a concentration of 2 mg/mL, were spotted in triplicate onto nitrocellulose membranes (GE Healthcare, Uppsala, Sweden). After a 30-minute drying period at room temperature, the membranes were blocked with MPBS buffer (140 mM NaCl, 10 mM Na2HPO4, 2.7 mM KCl, and 1.7 mM KH2PO4, pH 7.5) supplemented with 5% skim milk powder for 1 hour. Subsequently, the membranes were probed with either crude recombinant proteins (cell-free extracts) or purified recombinant proteins at a concentration of 50 μg/mL. Following water washes, the membranes were incubated with an HRP-conjugated (His)6-tag monoclonal antibody (Proteintech, IL, USA) for 2 hours. After another round of water washes, the membranes were developed for 2 minutes using an electrochemiluminescence solution.