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Standards of HA with gradient concentrations were used. A blank control and test samples were coated on 96-well microtiter plates (high binding microtiter plates, Coring, NY, USA) with 10 mM pH 7.4 phosphate-buffered saline (PBS), using 100 μL/well. The plates were incubated overnight at 4 °C. To wash the plates, PBS-T (PBS containing 0.5% Tween-20) was used five times with a microplate washer (Nanjing DeTie, Nanjing, China). Subsequently, blocking buffer (3% bovine serum albumin, BSA, with PBS-T) was added at 200 μL/well and incubated for 1 hour at 25 °C. After washing, SrCBM70-GST (5 μg/mL) was added at 100 μL/well and incubated for 1.5 hours at 25 °C. The plates were washed again and HRP-conjugated GST tag antibody (Proteintech, Illinois, USA) was added at a dilution of 5000-fold in PBS-T for 1.5 hours at 25 °C. Following the final wash step, 100 μL/well of 3,3',5,5'-tetramethylbenzidine (TMB) (Solarbio, Beijing, China) was added in the dark and incubated for 10 - 20 minutes. The reaction was stopped by adding 100 μL of 1 M HCl, resulting in a color change from blue to yellow. The absorbance was measured at 450 nm with a reference at 630 nm using a microplate reader (BIO-RAD, California, USA).