我希望你做一个 AI 写作导师你将担任学术论文拼写校对和修辞改进的角色。我会提供原文你会以更好学术更富有逻辑和精炼的英语回答我但确保意思不变请仅回答更正和改进的部分不要写解释：Enzyme-linked immunosorbent assay ELISA for quantitative detection is easy to implement and provides desirable s

Enzyme-linked immunosorbent assay (ELISA) is an easily implementable and specific method for quantitative detection (Engvall, 2010). The antibody, which specifically binds to the target molecule, plays a critical role in ELISA (Lai, Feldman & Clark, 2005). Despite a few existing reports (Hoffstetter, Griffin, Brown, Alan & Olson, 2018; Laura Werning et al., 2014), quantifying polysaccharides using ELISA remains challenging due to their poor immunogenicity and the difficulty in acquiring antibodies through animal immunity (Liners, Helbert & Van Cutsem, 2005).

Carbohydrate-binding module (CBM) is a domain found in carbohydrate active enzymes that recognizes and binds carbohydrate motifs (Shoseyov, Shani & Levy, 2006). CBM can be obtained through gene mining and heterologous expression, which is more efficient compared to antibodies obtained through animal immunity. Considering the polysaccharide recognition and binding capabilities of CBM, we hypothesized that CBM could be a potential substitute for antibodies in developing ELISA-like methods. Thus, we named these methods CBM-based ELISA methods, aiming to achieve simple and specific quantification of polysaccharides.

The objective of this study was to evaluate the feasibility of the CBM-based ELISA strategy. Our group recently discovered and characterized a hyaluronic acid-binding CBM protein called SrCBM70 (Mei et al., 2022). Hyaluronic acid (HA) is an economically important polysaccharide, composed of repeating disaccharide units [-4-D-glucuronic acid-β1-3-N-acetyl D-glucosamine-β1-]n (Rivas et al., 2022). Due to its various physicochemical properties and popular bioactivities (such as antiangiogenic, anti-inflammatory, immunosuppressive, and wound healing), HA has been extensively used in cosmetics, pharmaceuticals, future medicine, and functional food (Fallacara, Baldini, Manfredini & Vertuani, 2018). In this study, we established a CBM-based ELISA by using HA quantification as a specific issue and employing SrCBM70 with a GST tag as the recognition element instead of an antibody. We investigated and compared the performance of this method and discussed the applicability of CBM as an alternative to antibodies for simple and specific polysaccharide quantification.