It seems that there is an error in the code. The error message "行名长度不对" suggests that the length of the row names in the coldata object does not match the number of columns in the GBMdata object.

To fix this error, you need to make sure that the row names in coldata match the column names in GBMdata. You can check the column names of GBMdata by running colnames(GBMdata). Make sure that the row names in coldata are in the same order and have the same number of rows as the column names in GBMdata.

Once you have fixed the issue with coldata, you can proceed with the rest of the code to create the DESeqDataSet and perform DESeq analysis


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