How Crucial is Intermolecular Binding Affinity for Biologic Purification Yields and Purity?

The Impact of Intermolecular Binding Affinity on Biologic Purification

In the development of recombinant protein drugs, antibodies, ADCs, and other biologics, achieving high yields and purity during purification is paramount. Intermolecular binding affinity between the target molecule and the purification ligand plays a critical role in this process.

A strong binding affinity ensures efficient capture and separation of the desired product from impurities, leading to:

• Higher yields: Stronger binding minimizes loss of the target molecule during purification steps.* Increased purity: Efficient capture minimizes the co-purification of unwanted molecules, leading to a purer final product.

Therefore, selecting purification ligands with high affinity for the target molecule is crucial for optimizing downstream processing and ensuring the production of high-quality biologics.

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