High Binding Affinity in Purification: Is Higher Always Better?

Yes, generally speaking, a higher binding affinity between the target molecule and the purification ligand is desirable as it allows for more efficient capture and purification of the desired product. A higher affinity means that the target molecule has a stronger interaction with the purification ligand, leading to a higher likelihood of the target molecule binding to the ligand and being retained during purification processes. This can result in higher yields and purities of the desired product.