Experimental Techniques for Precise Intermolecular Binding Affinity Determination

To accurately calculate intermolecular binding affinity, several experimental tools can be utilized. Here are ten references in BibTeX format that provide insights into these methods:

1. Molecular dynamics simulations: @article{karplus2002molecular, title={Molecular dynamics simulations of biomolecules}, author={Karplus, Martin and McCammon, J Andrew}, journal={Nature structural biology}, volume={9}, number={9}, pages={646--652}, year={2002}, publisher={Nature Publishing Group} }

2. Isothermal titration calorimetry: @article{freire2009biophysical, title={Biophysical methods for the determination of protein--ligand binding constants}, author={Freire, Ernesto}, journal={Annual review of biophysics}, volume={38}, pages={123--142}, year={2009}, publisher={Annual Reviews} }

3. Surface plasmon resonance: @article{sauer2008surface, title={Surface plasmon resonance--a label-free tool for cellular analysis}, author={Sauer, Ursula G}, journal={Journal of biotechnology}, volume={133}, number={2}, pages={101--108}, year={2008}, publisher={Elsevier} }

4. Nuclear magnetic resonance spectroscopy: @article{ernst1990principles, title={Principles of nuclear magnetic resonance in one and two dimensions}, author={Ernst, Richard R and Bodenhausen, Geoffrey and Wokaun, Alexander}, journal={Principles of nuclear magnetic resonance in one and two dimensions}, volume={14}, year={1990}, publisher={Oxford university press} }

5. Fluorescence resonance energy transfer: @article{haas2001fluorescence, title={Fluorescence resonance energy transfer}, author={Haas, Edith and Katchalski-Katzir, Ephraim and Steinberg, Izhar Z}, journal={Journal of biochemical and biophysical methods}, volume={49}, number={1-3}, pages={1--16}, year={2001}, publisher={Elsevier} }

6. Microscale thermophoresis: @article{seidel2013microscale, title={Microscale thermophoresis quantifies biomolecular interactions under previously challenging conditions}, author={Seidel, Susanne A and Dijkman, Patricia M and Lea, Wendy A and van den Bogaart, Geert and Jerabek-Willemsen, Moran and Lazic, Ana and Joseph, Jeremiah S and Srinivasan, Prakash and Baaske, Philipp and Simeonov, Anton and others}, journal={Methods}, volume={59}, number={3}, pages={301--315}, year={2013}, publisher={Elsevier} }

7. Differential scanning fluorimetry: @article{pantoliano2001high, title={High-density miniaturized thermal shift assays as a general strategy for drug discovery}, author={Pantoliano, Michael W and Petrella, Elizabeth C and Kwasnoski, Jeffrey D and Lobanov, Victor S and Myslik, Jaroslaw and Graf, Eric and Carver, Thomas and Asel, Edward and Springer, Bryan A and Lane, Peter and others}, journal={Journal of biomolecular screening}, volume={6}, number={6}, pages={429--440}, year={2001}, publisher={SAGE Publications} }

8. X-ray crystallography: @article{drenth2007principles, title={Principles of protein X-ray crystallography}, author={Drenth, Jan}, journal={Springer Science & Business Media}, year={2007}, publisher={Springer} }

9. Mass spectrometry: @article{smith2002proteomics, title={Proteomics, genomics and transcriptomics: the integration of proteomics, genomics and transcriptomics using mass spectrometry}, author={Smith, Richard D and Anderson, Gordon A and Lipton, Mary S and Pasa-Tolic, Ljiljana and Shen, Yufeng and Conrads, Thomas P and Veenstra, Timothy D and Udseth, Harold R}, journal={Nature Reviews Genetics}, volume={3}, number={7}, pages={601--614}, year={2002}, publisher={Nature Publishing Group} }

10. Bio-layer interferometry: @article{rich2009biolayer, title={Biolayer interferometry for bioaffinity/biointeraction measurements}, author={Rich, Rebecca L and Myszka, David G}, journal={Methods in molecular biology}, volume={503}, pages={287--305}, year={2009}, publisher={Humana Press} }

These references provide comprehensive information on various experimental tools used for accurately calculating intermolecular binding affinity.