Boosting D-fructose-6-phosphate Hydrolase Activity: An In Silico Engineering Approach

Enhancing the activity of D-fructose-6-phosphate hydrolase is a significant pursuit in biotechnology, with implications for various industrial processes. This study employs an in silico engineering approach to achieve this goal, utilizing computational tools to gain insights into the enzyme's mechanism and identify potential targets for activity optimization. Through molecular dynamics simulations and other computational methods, we investigate the structural and dynamic features that govern enzyme activity. By analyzing the binding site and key residues involved in substrate recognition and catalysis, we propose targeted modifications to enhance the enzyme's catalytic efficiency. Our findings provide a theoretical basis for designing novel D-fructose-6-phosphate hydrolase variants with improved activity, paving the way for their application in biocatalysis and other biotechnological fields.