T4 DNA Ligase: Function, Applications & Molecular Biology

T4 DNA Ligase is an enzyme commonly used in molecular biology to join or 'ligate' DNA fragments together. It catalyzes the formation of a phosphodiester bond between adjacent nucleotides in double-stranded DNA, which is essential for the creation of recombinant DNA molecules. T4 DNA Ligase is often used in cloning experiments, where it is used to join together DNA fragments from different sources to create a new, hybrid DNA molecule. It is also used in other applications, such as the creation of gene fusions and the construction of synthetic genes.